A C-Type lectin collaborates with a CD45 phosphatase homolog to facilitate west nile virus infection of mosquitoes
Autor(es): Cheng, Gong; Cox, Jonathan; Wang, Penghua; Krishnan, Manoj N.; Dai, Jianfeng; Qian, Feng; Anderson, John F.; Fikrig, Erol
Resumo: West Nile virus (WNV) is the most common arthropod-borne flavivirus in the United States; however, the vector ligand(s) that participate in infection are not known. We now show that an Aedes aegypti C-type lectin, mosGCTL-1, is induced by WNV, interacts with WNV in a calcium-dependent manner, and facilitates infection in vivo and in vitro. A mosquito homolog of human CD45 in A. aegypti, designated mosPTP-1, recruits mosGCTL-1 to enable viral attachment to cells and to enhance viral entry. In vivo experiments show that mosGCTL-1 and mosPTP-1 function as part of the same pathway and are critical for WNV infection of mosquitoes. A similar phenomenon was also observed in Culex quinquefasciatus, a natural vector of WNV, further demonstrating that these genes participate in WNV infection. During the mosquito blood-feeding process, WNV infection was blocked in vivo with mosGCTL-1 antibodies. A molecular understanding of flaviviral-arthropod interactions may lead to strategies to control viral dissemination in nature.
Palavras-Chave: Mannose - Binding lectin; Protein - Tyrosine phosphatases ; Human ? Immunodeficiency ? Virus ; Dengue ? Virus ; Anopheles ? Gambiae ; Innate immunity ; Dendritic cell; Aedes aegypti ; Disease
Imprenta: Cell, v. 142, n. 5, p. 714-725, 2010
Identificador do objeto digital: 10.1016/j.cell.2010.07.038
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2010
