Advantages of intrathoracic inoculation of 'Aedes aegypti' for the isolation and reisolation of arboviruses
Autor(es): Gonzalez; Saluzzo; Herve
Resumo: Direct intrathoracic inoculation of Aedes aegypti with wild Central Africa mosquito pools was used for primary multiplication of viruses. The systematic use of this method permitted the isolation of 4 yellow fever strains in December 1978. The virus titration during the isolation exhibited a minimal increase of 3 log10 DL50 with this technique as compared to direct inoculation of suckling mice. The strains studied belonged to yellow fever, Zika, West-Nile, Orungo, Chikungunya and Bunyamwera serogroup viruses.
Palavras-Chave: Aedes aegypti; Animal experiment; Arthropod; Diagnosis; Methodology; Virus infection; Virus isolation; Yellow fever flavivirus; DANS
Imprenta: Annales de Virologie, v. 132, n. 4, p. 519-527, 1981.
Descritores: Zika virus - Zika fever ; Zika virus - Chikungunya Fever
Data de publicação: 1981
