Autor(es): Cecilia D,Kakade M,Alagarasu K,Patil J,Salunke A,Parashar D,Shah P S

Resumo: Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously detect and quantitate RNA of all dengue virus (DENV) serotypes and chikungunya virus (CHIKV). The sensitivity was 100 % for DENV and 95.8 % for CHIKV, whilst the specificity was 100 % for both viruses when compared with conventional RT-PCR. The detection limit ranged from 1 to 50 plaque-forming units. The assay was successfully used for differential diagnosis of dengue and chikungunya in Pune, where the viruses co-circulate.

Imprenta: Archives of Virology, v. 160, n. 1, p. 323-327, 2015

Descritores: Zika virus - Real Time PCR

Data de publicação: 2015

INDICADORES

ACESSO