Detection of chikungunya virus antigen by a novel rapid immunochromatographic test.
Autor(es): Okabayashi Tamaki,Sasaki Tadahiro,Masrinoul Promsin,Chantawat Nantarat,Yoksan Sutee,Nitatpattana Narong,Chusri Sarunyou,Morales Vargas Ronald E,Grandadam Marc,Brey Paul T,Soegijanto Soegeng,Mulyantno Kris Cahyo,Churrotin Siti,Kotaki Tomohiro,Faye Oumar,Faye Ousmane,Sow Abdourahmane,Sall Amadou Alpha,Puiprom Orapim,Chaichana Panjaporn,Kurosu Takeshi,Kato Seiji,Kosaka Mieko,Ramasoota Pongrama,Ikuta Kazuyoshi
Resumo: Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue fever. The present study aimed to develop a new immunochromatographic diagnosis test for the detection of chikungunya virus antigen in serum. Mice were immunized with isolates from patients with Thai chikungunya fever, East/Central/South African genotype, to produce mouse monoclonal antibodies against chikungunya virus. Using these monoclonal antibodies, a new diagnostic test was developed and evaluated for the detection of chikungunya virus. The newly developed diagnostic test reacted with not only the East/Central/South African genotype but also with the Asian and West African genotypes of chikungunya virus. Testing of sera from patients suspected to have chikungunya fever in Thailand (n = 50), Laos (n = 54), Indonesia (n = 2), and Senegal (n = 6) revealed sensitivity, specificity, and real-time PCR (RT-PCR) agreement values of 89.4%, 94.4%, and 91.1%, respectively. In our study using serial samples, a new diagnostic test showed high agreement with the RT-PCR within the first 5 days after onset. A rapid diagnostic test was developed using mouse monoclonal antibodies that react with chikungunya virus envelope proteins. The diagnostic accuracy of our test is clinically acceptable for chikungunya fever in the acute phase.
Imprenta: Journal of Clinical Microbiology, v. 53, n. 2, p. 382-388, 2015
Descritores: Zika virus - Real Time PCR
Data de publicação: 2015
