An approach towards peptide-based antibody detection for diagnosis of Chikungunya infection
Autor(es): Morey Shweta H,Kashyap Rajpal S,Purohit Hemant J,Taori Girdhar M,Daginawala Hatim F
Resumo: Chikungunya infection, although rarely fatal, is associated with significant morbidity which necessitates its diagnosis in the initial stages. Currently for diagnosis, together with clinical symptoms, immunological methods such as IgG/IgM detection, molecular methods such as real-time reverse transcriptase-polymerase chain reaction and viral isolation methods are available but they are either not very specific or they require high-level sophisticated infrastructures. In the present study, an enzyme-linked immunosorbent assay to evaluate antibody responses to peptides designed from the CHIKV E2 envelope glycoprotein was performed. Synthesized peptides were evaluated with confirmed Chikungunya and non-Chikungunya serum samples for antibody detection. The results demonstrate that the synthetic peptide-based diagnosis of Chikungunya can be an efficient and a more accessible approach in immunodiagnostics.
Imprenta: Biomarkers, v. 15, n. 6, p. 546-552, 2010
Descritores: Zika virus - Real Time PCR
Data de publicação: 2010
