Yellow fever monoclonal antibodies: Type-specific and cross-reactive determinants identified by immunofluorescence
Autor(es): Monath T.P., Schlesinger J.J., Brandriss M.W., Cropp C.B., Prange W.C.
Resumo: Monoclonal antibodies directed against the envelope glycoprotein and the NV3 non-structural viral protein of yellow fever (YF) were tested by the indirect fluorescent antibody technique against a variety of YF virus strains and heterologous flaviviruses. Monoclonal antibodies directed against the envelope glycoprotein exhibited YF strain-specificity, YF type-specificity, broad group cross-reactivity, or limited subgroup reactivity (YF + Banzi or YF + Koutango + Zika + Usutu + Uganda S). Monoclonal antibodies directed against NV3 reacted either with YF + Koutango or with YF + Banzi. These findings generally correlated with the results of biological tests reported previously. Monoclonal antibodies that were type-specific to YF will be useful for the rapid specific identification of YF virus isolates and are available from the Centers for Disease Control on request.
Palavras-Chave: Monoclonal Antibody; Virus Glycoprotein; Virus Protein; Immunofluorescence; In Vitro Study; Mouse; Nonhuman; Yellow Fever; Yellow Fever Flavivirus; Animals; Antibodies, Monoclonal; Antibodies, Viral; Antigens, Viral; Cross Reactions; Epitopes; Fluorescent Antibody Technique; Mice; Viral Envelope Proteins; Viral Proteins; Yellow Fever Virus; Flavivirus; Yellow Fever Virus
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 33, n. 4, p. 695-698, 1984.
Descritores: Zika virus - Proteins ; Zika virus - Antibodies ; Zika virus - Zika fever
Data de publicação: 1984
