Advantages of intrathoracic inoculation of 'Aedes aegypti' for the isolation and reisolation of arboviruses
Autor(es): Gonzalez J.P., Saluzzo J.F., Herve J.P.
Resumo: Direct intrathoracic inoculation of Aedes aegypti with wild Central Africa mosquito pools was used for primary multiplication of viruses. The systematic use of this method permitted the isolation of 4 yellow fever strains in December 1978. The virus titration during the isolation exhibited a minimal increase of 3 log10 DL50 with this technique as compared to direct inoculation of suckling mice. The strains studied belonged to yellow fever, Zika, West-Nile, Orungo, Chikungunya and Bunyamwera serogroup viruses.
Palavras-Chave: Aedes Aegypti; Animal Experiment; Arthropod; Diagnosis; Methodology; Virus Infection; Virus Isolation; Yellow Fever Flavivirus
Imprenta: Annales de Virologie, v. 132, n. 4, p.519-527, 1981.
Descritores: Zika virus - Arboviruses ; Zika virus - Chikungunya Fever ; Zika virus - Zika fever
Data de publicação: 1981
