One-step RT-PCR for detection of Zika virus
Autor(es): Faye O., Faye O., Dupressoir A., Weidmann M., Ndiaye M., Alpha Sall A.
Resumo: Background: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. Objective: To develop a one-step RT-PCR assay to detect ZIKV in human serum. Study design: An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. Results: The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. Conclusions: The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples. © 2008 Elsevier B.V. All rights reserved.
Palavras-Chave: Flavivirus, reverse transcription polymerase chain reaction, zika flavivirus
Imprenta: Journal of Clinical Virology, v. 43, n. 1, p. 96-101, sept. 2008
Descritores: Zika virus - Cell ; Zika virus - RNA ; Zika virus - Serology ; Zika virus - PCR detection ; Zika virus - RNA virus ; Zika virus - RT-PCR ; Zika virus - Zika fever
Data de publicação: 2008
