A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein
Autor(es): Anzhong Li; Jingyou Yu; Mijia Lu; Yuanmei Ma; Attia, Zayed; Shan, Chao; Miaoge Xue; Liang, Xueya; Craig, Kelsey; Makadiya, Nirajkumar; Jennifer J. He; Jennings, Ryan; Pei-Yong Shi; Peeples, Mark E.; Shan-Lu Liu; Boyaka, Prosper N.; Jianrong Li
Resumo: Current efforts to develop Zika virus (ZIKV) subunit vaccines have been focused on pre-membrane (prM) and envelope (E) proteins, but the role of NS1 in ZIKV-specific immune response and protection is poorly understood. Here, we develop an attenuated recombinant vesicular stomatitis virus (rVSV)-based vaccine expressing ZIKV prM-E-NS1 as a polyprotein. This vectored vaccine candidate is attenuated in mice, where a single immunization induces ZIKV-specific antibody and T cell immune responses that provide protection against ZIKV challenge. Co-expression of prM, E, and NS1 induces significantly higher levels of Th2 and Th17 cytokine responses than prM-E. In addition, NS1 alone is capable of conferring partial protection against ZIKV infection in mice even though it does not induce neutralizing antibodies. These results demonstrate that attenuated rVSV co-expressing prM, E, and NS1 is a promising vaccine candidate for protection against ZIKV infection and highlights an important role for NS1 in ZIKV-specific cellular immune responses
Imprenta: Nature Communications, v. 9, n. 3067, 2018
Descritores: Zika virus - Vaccine
Data de publicação: 2018
