Field-deployable viral diagnostics using CRISPR-Cas13

Autor(es): Myhrvold, Cameron; Freije, Catherine A.; Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Met, Hayden C.

Resumo: Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENV detection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015-2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms

Imprenta: Science, v. 360, n. 6387, p. 444-448, 2018

Identificador do objeto digital: 10.1126/science.aas8836

Descritores: Zika virus - Cell

Data de publicação: 2018