Putative dengue virus receptors from mosquito cells.

Autor(es): Muñoz M L; Cisneros A; Cruz J; Das P; Tovar R; Ortega A

Resumo: Dengue viruses are arthropod-borne, single-stranded RNA viruses. Aëdes aegypti and Aëdes albopictus are the principal vectors. In order to understand the molecular basis of dengue virus infections we explored the biochemical identity of dengue-2 (DEN-2) virus receptors in the Aëdes albopictus-derived cell line C6/36. We show here that DEN-2 interacts with two major polypeptides of 80 and 67 kDa. Polyclonal anti-C6/36 membrane antibodies block DEN-2 binding to intact C6/36 monolayers as well as to membrane extracts. Our results strongly suggest that the identified polypeptides are part of the DEN-2 receptors.

Palavras-Chave: Dengue virus; Receptor; C6/36 cell

Imprenta: FEMS Microbiology Letters, v. 168, n. 2, p. 251-258, 1998

Identificador do objeto digital: 10.1111/j.1574-6968.1998.tb13281.x

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Antibodies ; Aedes aegypti - virus ; Aedes aegypti - Dengue ; Aedes aegypti - Immunology

Data de publicação: 1998