Protocol for dengue infections in mosquitoes (A. aegypti) and infection phenotype determination.

Autor(es): Das Suchismita; Garver Lindsey; Ramirez Jose Ruiz; Xi Zhiyong; Dimopoulos George

Resumo: The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of the virus in the mosquito tissues. This protocol is routinely used for studying mosquito-virus interactions, especially for identification of novel host factors that are able to determine vector competence. The entire experiment must be conducted in a BSL2 laboratory. Similar to Plasmodium falciparum infections, proper attire including gloves and lab coat must be worn at all times. After the experiment, all the materials that came in contact with the virus need to be treated with 75% ethanol and bleached before proceeding with normal washing. All other materials need to be autoclaved before discarding them.

Palavras-Chave: Cellular Biology; Issue 5; Mosquito; Dengue; Fever; Infectious disease

Imprenta: Journal of Visualized Experiments, v. 5, n. 220, 2007

Identificador do objeto digital: 10.3791/220

Descritores: Aedes aegypti - Flaviviridae ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Viral infections ; Aedes aegypti - virus ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2007