Proteomic analysis of the mosquito Aedes aegypti midgut brush border membrane vesicles.
Autor(es): Popova-Butler Alexandra; Dean Donald H
Resumo: We analyzed brush border membrane vesicle proteins from isolated midguts of the mosquito Aedes aegypti, by two proteomic methods: two-dimensional gel electrophoresis (isoelectric focusing and SDS-PAGE) and a shotgun two-dimensional liquid chromatographic (LS/LS) approach based on multidimensional protein identification technology (MudPIT). We were interested in the most abundant proteins of the apical brush border midgut membrane. About 400 spots were detected on 2D gels and 39 spots were cored and identified by mass spectrometry. 86 proteins were identified by MudPIT. Three proteins, arginine kinase, putative allergen and actin are shown to be the most predominant proteins in the sample. The total number of 36 proteins detected by both methods represents the most abundant proteins in the BBMV.
Palavras-Chave: Midgut proteome; Mosquito
Imprenta: Journal of Insect Physiology, v. 55, n. 3, p. 264-272, 2009
Identificador do objeto digital: 10.1016/j.jinsphys.2008.12.008.
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Cell ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Molecular methods
Data de publicação: 2009
