Persistence of viral RNA in chikungunya virus-infected Aedes aegypti (Diptera: Culicidae) mosquitoes after prolonged storage at 28°C.
Autor(es): Mavale Mangala; Sudeep Anakkathil; Gokhale Mangesh; Hundekar Supriya; Parashar Deepti; Ghodke Youwaraj; Arankalle Vidya; Mishra Akhilesh Chandra
Resumo: Experiments were conducted to determine the persistence of chikungunya viral (CHIKV) RNA in experimentally infected Aedes aegypti mosquitoes stored for prolonged periods at 28°C. Intra-thoracically inoculated mosquitoes with confirmed positivity were killed by quick freezing at -80°C, applied to sticky tape, and stored at 28°C with 80 ? 5% relative humidity (RH). At weekly intervals, five mosquitoes were removed from the tape randomly and assayed individually for detection of viral RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). CHIKV RNA was detected up to 12 weeks in dry mosquitoes by RT-PCR. Virus could not be isolated either in cell culture or in the suckling Swiss-albino mouse system at any stage. This study demonstrated the persistence of CHIKV viral RNA up to 12 weeks when stored at 28°C with RH 80 ? 5%. This finding will have significance in CHIKV surveillance programs in mosquito populations or field-based studies in countries where maintenance of a cold chain is a concern.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 86, n. 1, p. 178-180, 2012
Identificador do objeto digital: 10.4269/ajtmh.2012.11-0236
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - virus ; Aedes aegypti - Public health
Data de publicação: 2012
