Peroxiredoxin 5 from common cutworm (Spodoptera litura) acts as a potent antioxidant enzyme.
Autor(es): Wan Hu; Kang Tinghao; Zhan Sha; You Hong; Zhu Fuxing; Lee Kwang Sik; Zhao Haigang; Jin Byung Rae; Li Jianhong
Resumo: In this study, we describe the cloning and characterization of a Prx from the common cutworm Spodoptera litura (SlPrx5). The SlPrx5 cDNA contains an open reading frame of 477 bp encoding a predicted protein of 159 amino acid residues, 16.902 kDa, and an isoelectric point of 7.68. Furthermore, the deduced amino acid sequence of the SlPrx5 cDNA showed 86% identity to Papilio xuthus Prx5, 72% to Aedes aegypti Prx5, and 64-67% to other insect Prxs. A phylogenetic analysis further revealed that the deduced amino acid sequence of SlPrx5 groups within the atypical 2-Cys Prx cluster. Recombinant SlPrx5 (20 kDa) purified from baculovirus-infected insect cells was found to reduce H2O2 in the presence of electrons donated by dithiothreitol and protect super-coiled DNA from damage by metal-catalyzed oxidation in vitro. During S. litura development, SlPrx5 is constitutively expressed in the epidermis, fat body, and midgut, with the highest expression occurring in the sixth-instar larval stage in the fat body and midgut. Additionally, SlPrx5 mRNA expression was up-regulated after injection with H2O2, cumene hydroperoxide, indoxacarb, and metaflumizone. A disc diffusion assay indicated that recombinant SlPrx5 can play a functional role in protecting cells from oxidative stress in vivo. These results provide insight into the role of SlPrx5 during development and the oxidative stress response of S. litura.
Palavras-Chave: Antioxidant enzyme; Spodoptera litura; Common cutworm; Oxidative stress; Peroxiredoxin
Imprenta: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, v. 175, p. 53-61, 2014
Identificador do objeto digital: 10.1016/j.cbpb.2014.06.008.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins
Data de publicação: 2014
