Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0).
Autor(es): Yamao Takuya; Eshita Yuki; Kihara Yuki; Satho Tomomitsu; Kuroda Makoto; Sekizuka Tsuyoshi; Nishimura Miho; Sakai Kouji; Watanabe Shumpei; Akashi Hiroomi; Rongsriyam Yupha; Komalamisra Narumon; Srisawat Raweewan; Miyata Takeshi; Sakata Akira; Hosokawa Masato; Nakashima Manabu; Kashige Nobuhiro; Miake Fumio; Fukushi Shuetsu; Nakauchi Mina; Saijo Masayuki; Kurane Ichiro; Morikawa Shigeru; Mizutani Tetsuya
Resumo: In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae.
Palavras-Chave: West Nile Virus; Rice Stripe Virus; Rift Valley Fever Virus; Japanese Encephalitis Virus; Mosquito Cell Line
Imprenta: Archives of Virology, v. 154, n. 1, p. 153-158, 2009
Identificador do objeto digital: 10.1007/s00705-008-0285-5.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Molecular methods ; Aedes aegypti - virus
Data de publicação: 2009
