Neoaplectana carpocapsae:encapsulation in Aedes aegypti and changes in host hemocytes and hemolymph proteins.

Autor(es): Andreadis T G; Hall D W

Resumo: Following encapsulation of a nematode parasite Neoaplectana carpocapsae by larval Aedes aegypti, there were significant decreases in both the total hemocyte count and in the number of DOPA-oxidase positive hemocytes within the anal papillae. Ligation experiments indicate these hemocytes are not necessary for successful capsule formation. The possibility of these changes resulting from a pathological condition created by the parasite are discussed. Disc electrophoresis revealed several changes in the protein migration pattern of A. aegypti hemolymph following encapsulation of N. carpocapsae. Gels stained with amido schwartz showed a shift in certain bands, a reduction in intensity of another, and the presence of an additional protein fraction. These changes appear to be specific for parasitism and/or encapsulation. Incubation of gels in DOPA solution revealed an increased intensity of one protein fraction which is not specific for encapsulation but may be the result of a wound response. It appears that some protein is released by the host or by the parasite in response to parasitism. While the function of this protein is unknown, it may play a role in the defense reactions of the host.

Palavras-Chave: Aedes aegypti; Neoaplectana carpocapsae; Encapsulation; Melanization; Hemocytes; Hemolymph proteins; DOPA-oxidase

Imprenta: Experimental Parasitology, v. 39, n. 2, p. 252-261, 1976

Identificador do objeto digital: 10.1016/0014-4894(76)90125-9

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins

Data de publicação: 1976