nanos gene control DNA mediates developmentally regulated transposition in the yellow fever mosquito Aedes aegypti.

Autor(es): Adelman Zach N; Jasinskiene Nijole; Onal Sedef; Juhn Jennifer; Ashikyan Aurora; Salampessy Michael; MacCauley Todd; James Anthony A

Resumo: Transposable elements (TEs) are proposed as a basis for developing drive systems to spread pathogen resistance genes through vector mosquito populations. The use of transcriptional and translational control DNA elements from genes expressed specifically in the insect germ line to mediate transposition offers possibilities for mitigating some of the concerns about transgene behavior in the target vector species and eliminating effects on nontarget organisms. Here, we describe the successful use of the promoter and untranslated regions from the nanos (nos) orthologous gene of the yellow fever mosquito, Aedes aegypti, to control sex- and tissue-specific expression of exogenously derived mariner MosI transposase-encoding DNA. Transgenic mosquitoes expressed transposase mRNA in abundance near or equal to the endogenous nos transcript and exclusively in the female germ cells. In addition, MosI mRNA was deposited in developing oocytes and localized and maintained at the posterior pole during early embryonic development. Importantly, four of five transgenic lines examined were capable of mobilizing a second MosI transgene into the mosquito genome, indicating that functional transposase was being produced. Thus, the nos control sequences show promise as part of a TE-based gene drive system.

Palavras-Chave: Dengue; Gene drive; Transposon

Imprenta: Proceedings of the National Academy of Sciences of the United States of America, v. 104, n. 24, p. 9970-9975, 2007

Identificador do objeto digital: 10.1073/pnas.0701515104

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Viral infections

Data de publicação: 2007