Morphology and behaviour of cultured Aedes aegypti mosquito cells.
Autor(es): Peleg J; Shahar A
Resumo: Cultured Aeaes aegyprt mosqmro ceus mspiay tnree msunct growth stages. First a monolayer is formed, then some of the cells aggregate, and finally there appear vesicular bodies. Cells composing the aggregates and vesicles are endowed with the capacity to produce secretory substances such as melanin, and a chitin-like substance. These properties are retained by the cells even after over 170 passages in vitro. In previous communications on the growth of cultured AYdes aegypti mosquito cells we reported development of differentiated vesicle-like structures (Peleg and Shahar, 1969; Peleg, 1969). In distinction from the vesicles which were observed in primary cultures of larval tissues of A~?tes ae~,9'pti (Bhat and Singh, 1969) the vesicles to be described here continue to develop even after over 170 subcultures of the cells. In this communication we report on : (1) The growth stages leading to the formation of vesicles. (2) The morphology of the vesicles as revealed by light, phase contrast and electron microscopy, and (3) The capacity of the cells organized in vesicles to produce secretory substances.
Imprenta: Tissue & Cell, v. 4, n. 1, p. 55-62, 1972
Identificador do objeto digital: 10.1016/S0040-8166(72)80006-5
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Cell
Data de publicação: 1972
