Molecular cloning and expression of two hexamerin cDNAs from the mosquito, Aedes aegypti.

Autor(es): Gordadze A V; Korochkina S E; Zakharkin S O; Norton A L; Benes H

Resumo: Fourth-instar larvae of Aedes aegypti synthesize two types of hexamerins, Hexamerin-1 (AaHex-1) and Hexamerin-2 (AaHex-2), whose subunits are distinguished by different methionine and aromatic amino acid contents. In early female pupae only the methionine-rich AaHex-1gamma subunit accumulates to two-fold higher levels than in males. To investigate the relationship between hexamerin structure and the roles of Hex-1 and Hex-2 during mosquito development and reproduction, we have cloned and sequenced cDNAs encoding the AaHex-2alpha, -2beta and AaHex-1gamma subunits. Comparison with other insect hexamerins revealed that the Aedes Hex-1 and Hex-2 proteins belong, respectively, to the two hexamerin subfamilies previously defined for brachyceran Diptera. Probes specific for the Hex-2alpha and Hex-1gamma transcripts showed that expression of both genes follows the same developmental timetable. However, greater Hex-1gamma mRNA accumulation may contribute to the higher levels of Hex-1 gamma protein in early female pupae.

Imprenta: Insect Molecular Biology, v. 8, n. 1, p. 55-66, 1999

Identificador do objeto digital: 10.1046/j.1365-2583.1999.810055.x

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins

Data de publicação: 1999