Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA.

Autor(es): Rayms-Keller A; McGaw M; Oray C; Carlson J O; Beaty B J

Resumo: We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine-rich domains, two of which flank a proline/serine/threonine-rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal-responsive gene from an aquatic insect.

Imprenta: Insect Molecular Biology, v. 9, n. 4, p. 419-426, 2000

Identificador do objeto digital: 10.1046/j.1365-2583.2000.00202.x

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins

Data de publicação: 2000