Microsatellite markers characterized in the mosquito Aedes taeniorhynchus (Diptera, Culicidae), a disease vector and major pest on the American coast and the Gal?!pagos Islands.
Autor(es): Bataille Arnaud; Horsburgh Gavin J; Dawson Deborah A; Cunningham Andrew A; Goodman Simon J
Resumo: The black salt-marsh mosquito, Aedes taeniorhynchus, plays an important role in the transmission of arboviruses such as West Nile virus and other pathogens of concern for human and animal health in North and Latin America. This mosquito is notably the only widely distributed mosquito species found in the Gal?!pagos Islands, where its impact as disease vector has not yet been studied. The use of microsatellite markers can significantly improve our understanding of the population structure and dynamics of A. taeniorhynchus and its role in the transmission of diseases. Here we report the isolation of 12 unique microsatellite loci using an enrichment protocol. We also identified other multi-locus microsatellites linked to transposable elements. The presence of such elements may explain why the isolation of useful scorable microsatellite markers in the Aedes genus is often difficult. Four of the markers isolated amplified polymorphic products in Aedes aegypti, Aedes albopictus and/or Aedes japonicus.
Palavras-Chave: Aedes taeniorhynchus; Disease vector; Arbovirus; Microsatellite; Transposable element; Cross-amplification; Galápagos Islands
Imprenta: Infection, Genetics and Evolution, v. 9, n. 5, p. 971-975, 2009
Identificador do objeto digital: 10.1016/j.meegid.2009.04.003
Descritores: Aedes aegypti - Pathogenesis ; Aedes aegypti - Virus ; Aedes aegypti - Arbovirus ; Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Viral infections ; Aedes aegypti - Transmission ; Aedes aegypti - Public health
Data de publicação: 2009