Microinjection of A. aegypti embryos to obtain transgenic mosquitoes.
Autor(es): Jasinskiene Nijole; Juhn Jennifer; James Anthony A
Resumo: In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.
Palavras-Chave: Cellular Biology; Mosquito; Malaria; Virus; Dengue; Genetics; RNAi; Transgenic; Microinjections
Imprenta: Journal of visualized experiments, v. 5, n. 219, 2007
Identificador do objeto digital: 10.3791/219
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Viral infections ; Aedes aegypti - Molecular methods ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2007
