Microinjection of A. aegypti embryos to obtain transgenic mosquitoes.

Autor(es): Jasinskiene Nijole; Juhn Jennifer; James Anthony A

Resumo: In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.

Palavras-Chave: Cellular Biology; Mosquito; Malaria; Virus; Dengue; Genetics; RNAi; Transgenic; Microinjections

Imprenta: Journal of visualized experiments, v. 5, n. 219, 2007

Identificador do objeto digital: 10.3791/219

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Viral infections ; Aedes aegypti - Molecular methods ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2007