Metabolism of Aedes aegypti cells grown in vitro. I. Incorporation of 3 H-uridine and 14 C-leucine.
Autor(es): Hayashi Y; Soi S S
Resumo: Metabolic activity ofA. aegypti cells grown in vitro has been studied by incorporation of3H-uridine and14C-leucine. "Chase" experiments with unlabeled precursors, and the use of actinomycin D and puromycin, showed that3H-uridine was incorporated into cellular RNA, and that14C-leucine was incorporated into protein of these cells. Incorporation of3H-uridine was inhibited when actinomycin D was used at a concentration of 10 ?g/ml, and14C-leucine incorporation was inhibited to the same extent by puromycin at a concentration of 100 ?g/ml medium.
Palavras-Chave: Actinomycin; Puromycin; Erythrosin; Aedes AEGYPTI; Ehrlich Ascites Cell
Imprenta: In Vitro, v. 6, n. 2, p. 148- 152, 1970
Identificador do objeto digital: 10.1007/BF02616116
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Protein synthesis ; Aedes aegypti - RNA
Data de publicação: 1970
