Isolation of novel Bacillus species showing high mosquitocidal activity against several mosquito species.

Autor(es): Hayes Sabrina R; Hudon Michael; Park Hyun-Woo

Resumo: Two novel mosquitocidal bacteria, VB17 and VB24, identified as new Bacillus species were isolated from dead mosquito larvae obtained in Florida aquatic habitats. Gas chromatographic analysis of fatty acid methyl esters (GC-FAME) and 16S rRNA sequencing indicated that VB24 is closely related to Bacillus sphaericus whereas VB17 does not have a close relationship with either Bacillus thuringiensis or B. sphaericus. Both isolates were significantly more active than B. sphaericus 2362 against Aedes taeniorhynchus, Anopheles quadrimaculatus, Culex quinquefasciatus larvae, and as active as B. sphaericus 2362 against Anopheles gambiae. Interestingly, however, both were not active against Aedes aegypti larvae, indicating some level of insecticidal specificity.

Palavras-Chave: Bacillus sp.; Mosquitocidal; GC-FAME; LC-MS/MS

Imprenta: Journal of Invertebrate Pathology, v. 107, n. 1, p. 79-81, 2011

Identificador do objeto digital: 10.1016/j.jip.2011.01.005

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Transmission ; Aedes aegypti - Public health

Data de publicação: 2011