Interaction of the monoxenic trypanosomatid Blastocrithidia culicis with the Aedes aegypti salivary gland.

Autor(es): Nascimento Michelle Tanny C; Garcia Maria Carolina F; da Silva Karen Pereira; Pinto-da-Silva Lucia Helena; Atella Georgia C; Motta Maria Cristina M; Saraiva Elvira M

Resumo: Blastocrithidia culicis is a monoxenous trypanosomatid that inhabits mosquitoes. Although its life cycle in these insects has not been described, we recently demonstrated that B. culicis colonizes the Aedes aegypti digestive tract and is also able to reach the mosquito's haemocoel. In this work, we describe the interaction of B. culicis with the A. aegypti salivary gland in vitro and in vivo. Ultrastructural analysis reveals different steps of the invasion process, beginning with the insertion of the B. culicis anterior flagellum into the basal lamina of the gland and ending with the protozoan inside the salivary gland acini compartment. Carbohydrates are involved in the initial adhesion of B. culicis cells to the salivary glands, as demonstrated by protozoan binding inhibition assays and fluorescent lectin labeling of the trypanosomatid-salivary gland interaction. B. culicis is able to survive after incubation in vitro in the mosquito haemolymph, and trypanosomatid binding to salivary glands was confirmed by the injection of radioactively labeled protozoa into the mosquito haemocoel. These results suggest that salivary gland invasion could be part of the B. culicis life cycle in A. aegypti, raising the possibility that B. culicis can be transmitted by these mosquitoes.

Palavras-Chave: Blastocrithidia; Aedes aegypti; Mosquito salivary gland carbohydrates; Mosquito salivary gland lectin; Protozoa - Insect interaction; Monoxenic trypanosomatid

Imprenta: Acta Tropica, v. 113, n. 3, p. 269-278, 2010

Identificador do objeto digital: 10.1016/j.actatropica.2009.11.010

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Transmission

Data de publicação: 2010