Insights into the role of gp63-like proteins in lower trypanosomatids.

Autor(es): Masini d'Avila-Levy Claudia; de Almeida Dias Felipe; Nogueira de Melo Ana Cristina; Martins Juliana Lopes; De Carvalho Santos Lopes Angela Hampshire; Souza Dos Santos André Luis; Vermelho Alane Beatriz; Branquinha Marta Helena

Resumo: Any actual understanding of trypanosomatids in general requires a comprehensive analysis of the less-specialized species as thorough as our knowledge of the more specialized Leishmania and Trypanosoma. In this context, we have shown by antibody cross-reactivity that purified extracellular metallopeptidases from Phytomonas françai, Crithidia deanei (cured strain) and Crithidia guilhermei share common epitopes with the leishmanial gp63. Flow cytometry and fluorescence microscopy analyses indicated the presence of gp63-like molecules on the cell surface of these lower trypanosomatids. Binding assays with explanted guts of Aedes aegypti incubated with purified gp63 and the pretreatment of trypanosomatids with anti-gp63 antibodies indicated that the gp63-like molecules are involved in the adhesive process of these trypanosomatids to the A. aegypti gut wall. In addition, our results indicate for the first time that the gp63-like molecule binds to a polypeptide of 50 kDa on the A. aegypti gut epithelium extract.

Palavras-Chave: Trypanosomatids; Gp63; Cellular interaction, Aedes aegypti, Phytomonas, Crithidia

Imprenta: FEMS Microbiology Letters, v. 254, n. 1, p. 149-156, 2006

Identificador do objeto digital: 10.1111/j.1574-6968.2005.00022.x

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Antibodies

Data de publicação: 2006