Injection of dsRNA into female A. aegypti mosquitos.
Autor(es)Luna Brian M; Juhn Jennifer; James Anthony A A
ResumoReverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
ImprentaJournal of Visualized Experiments, v. 5, p. 215, 2007
Identificador do Objeto Digital10.3791/215
DescritoresAedes aegypti - Biochemistry ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Molecular methods ; Aedes aegypti - Virus ; Aedes aegypti - Dengue
Data de Publicação:2007
