Injection of dsRNA into female A. aegypti mosquitos.

Autor(es): Luna Brian M; Juhn Jennifer; James Anthony A A

Resumo: Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Imprenta: Journal of Visualized Experiments, v. 5, p. 215, 2007

Identificador do objeto digital: 10.3791/215

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Molecular methods ; Aedes aegypti - Virus ; Aedes aegypti - Dengue

Data de publicação: 2007