Induction of apoptosis in densovirus infected Aedes aegypti mosquitoes.

Autor(es): Roekring Songsak; Smith Duncan R

Resumo: The mechanism of death in densovirus infected mosquitoes remains unexplored. This study investigated the cellular consequences of densovirus infection in Aedes aegypti mosquitoes after a second generation challenge with a densovirus isolated from adult Aedes albopictus mosquitoes in Thailand (AThDNV). Specimens were analyzed by TUNEL assay, fluorescent in situ hybridization (FISH) and a calorimic assay to detect activation of caspase 3-like activity. After challenge, moribund mosquitoes showed considerable evidence of TUNEL positive cells. The caspase 3-like activity assay showed that the presence of TUNEL positive cells was associated with increased levels of activated caspase 3-like activity in AThDNV infected mosquitoes.

Palavras-Chave: Aedes; Apoptosis; Brevidensovirus; Caspase; Densovirus

Imprenta: Journal of Invertebrate Pathology, v. 104, n. 3, p. 239-241, 2010

Identificador do objeto digital: 10.1016/j.jip.2010.04.002. 

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Cytopathology ; Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Public health

Data de publicação: 2010