In vitro expression and pharmacology of the 5-HT7-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves.

Autor(es): Lee D W; Pietrantonio P V

Resumo: We have previously reported the cloning of a 5-hydroxytryptamine receptor (Aedes 5-HT7-like receptor) from adult Aedes aegypti. For functional expression of the Aedes 5-HT7-like receptor, CHO-K1 cells were stably transfected with a receptor expression construct, pC5-HT7. The Aedes 5-HT7-like receptor positively coupled to Gs protein, increasing intracellular cAMP in response to 5-HT; adenylyl cyclase activity was induced in a concentration-dependent, saturable manner. Only 5-HT, and not octopamine, dopamine or tyramine, caused the induction of cAMP. At 10 nM 5-HT a weak synergism was observed between octopamine and 5-HT. Other known agonists of the mammalian 5-HT7 receptor were tested. Their order of potency was: 5-HT > 5-CT = 8-OH-DPAT > pimozide. This is the first report on the functional expression of a mosquito neurohormone receptor.

Imprenta: Insect Molecular Biology, v. 12, n. 6, p. 561-569, 2003

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Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Protein synthesis ; Aedes aegypti - Proteins ; Aedes aegypti - RT-PCR

Data de publicação: 2003