Improved accuracy of the transcriptional profiling method of age grading in Aedes aegypti mosquitoes under laboratory and semi-field cage conditions and in the presence of Wolbachia infection.
Autor(es): Caragata E P; Poinsignon A; Moreira L A; Johnson P H; Leong Y S; Ritchie S A; O'Neill S L; McGraw E A
Resumo: Transcriptional profiling is an effective method of predicting age in the mosquito Aedes aegypti in the laboratory, however, its effectiveness is limited to younger mosquitoes. To address this we used a microarray to identify new gene candidates that show significant expression changes in older mosquitoes. These genes were then used to create a revised model, which upon evaluation in both laboratory and semi-field conditions, proved to have improved accuracy overall and for older mosquitoes. In association with the development of symbiont-based control strategies for Ae. aegypti, we also tested the model's accuracy for Wolbachia-infected mosquitoes and found no decline in performance. Our findings suggest that the new model is a robust and powerful tool for age determination in Australian Ae. aegypti populations.
Imprenta: Insect Molecular Biology, v. 20, n. 2, p. 215-224, 2011
Identificador do objeto digital: 10.1111/j.1365-2583.2010.01059.x.
Descritores: Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis
Data de publicação: 2011
