Immunohistochemical analysis of protein expression during Aedes aegypti development.

Autor(es): Clemons Anthony; Flannery Ellen; Kast Kristopher; Severson David; Duman-Scheel Molly

Resumo: Blood-feeding mosquitoes, Including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and gene products that regulate development are of particular interest. This protocol for immunohistochemical analysis of protein expression can be used to analyze expression of developmental proteins of interest in Ae. aegypti embryos, larvae, and pupae, which will be critical for the development of markers for particular developing tissues.

Imprenta: Cold Spring Harbor protocols, v. 10, pdb. prot5510, 2010

Identificador do objeto digital: 10.1101/pdb.prot5510.

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Genome ; Aedes aegypti - Proteins ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemic ; Aedes aegypti - Public health

Data de publicação: 2010