Identification and expression profile analysis of putative odorant-binding proteins in Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae).
Autor(es): Gong Zhong-Jun; Miao Jin; Duan Yun; Jiang Yue-Li; Li Tong; Wu Yu-Qing
Resumo: Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play important roles in the olfactory recognition. The orange blossom midge, Sitodiplosis mosellana is a cereal specialist, and utilizes pheromone and host odorant as a cue for its mating and oviposition. However, OBP genes have not been largely identified in S. mosellana. Based on the sequenced transcriptome database, twenty-six OBP genes were identified in S. mosellana for the first time. Phylogenetic analysis revealed that S. mosellana OBP genes are more closely related to Mayetiola destructor OBP genes than to Aedes aegypti OBP genes. Most OBP genes seemed to be antenna-specific, but differentially expressed in male and female antennae. Three OBP genes (OBP9, OBP19 and OBP23) are leg-specific. And also, most OBP genes have higher expression levels in adults. Only one OBP gene (OBP10) has higher expression levels in larval stages. These findings serve as an important basis for understanding the molecular mechanisms of chemosensory perception.
Palavras-Chave: Amino Acid Sequence Animals Base Sequence Diptera/genetics Diptera/growth & development Female Gene Expression Regulation; Developmental Insect Proteins/classification Insect Proteins/genetics Male Molecular Sequence Data Multigene Family Phylogeny Protein Isoforms/genetics Receptors; Odorant/classification Receptors; Odorant/genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Homology; Amino Acid Sequence Homology; Nucleic Acid Sex Factors Transcriptome
Imprenta: Biochemical and Biophysical Research Communications. v. 444, n. 2, p. 164-170, 2014
Identificador do objeto digital: 10.1016/j.bbrc.2014.01.036
Descritores: Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Real Time PCR ; Aedes aegypti - RT-PCR ; Aedes aegypti - Public health
Data de publicação: 2014
