Identification and characterization of a novel peritrophic matrix protein, Ae-Aper50, and the microvillar membrane protein, AEG12, from the mosquito, Aedes aegypti.

Autor(es): Shao Li; Devenport Martin; Fujioka Hisashi; Ghosh Anil; Jacobs-Lorena Marcelo

Resumo: Immuno-screening of an adult Aedes aegypti midgut cDNA expression library with anti-peritrophic matrix antibodies identified cDNAs encoding a novel peritrophic matrix protein, termed Ae. aegypti Adult Peritrophin 50 (Ae-Aper50), and the epithelial cell-surface membrane protein, AEG12. Both genes are expressed exclusively in the midguts of adult female mosquitoes and their expression is strongly induced by blood feeding. Ae-Aper50 has a predicted secretory signal peptide and five chitin-binding domains with intervening mucin-like domains. Localization of Ae-Aper50 to the peritrophic matrix was demonstrated by immuno-electron microscopy. Recombinant Ae-Aper50 expressed in baculovirus-infected insect cells binds chitin in vitro. Site-directed mutagenesis was used to study the role that cysteine residues from a single chitin-binding domain play in the binding to a chitin substrate. Most of the cysteine residues proved to be critical for binding. AEG12 has a putative secretory signal peptide at the amino-terminus and a putative glycosyl-phosphatidylinositol (GPI) anchor signal at its carboxyl-terminus and the protein was localized by immuno-electron microscopy to the midgut epithelial cell microvilli.

Palavras-Chave: Mosquito vector; Mosquito larva; Nontarget organism; Toxicity assay; Insecticidal protein

Imprenta: Insect Biochemistry and Molecular Biology, v. 35, n. 9, p. 947-959, 2005

Identificador do objeto digital: 10.1007/s00436-013-3658-y

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Protein synthesis ; Aedes aegypti - Proteins ; Aedes aegypti - Antibodies ; Aedes aegypti - Molecular screening

Data de publicação: 2005