Germline excision of transgenes in Aedes aegypti by homing endonucleases.

Autor(es): Aryan Azadeh; Anderson Michelle A E; Myles Kevin M; Adelman Zach N

Resumo: Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.

Imprenta: Scientific Reports, v. 3, n. 1603, 2013

Identificador do objeto digital: 10.1038/srep01603

Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Virus ; Aedes aegypti - Dengue

Data de publicação: 2013