Genetic mapping a meiotic driver that causes sex ratio distortion in the mosquito Aedes aegypti.

Autor(es): Shin Dongyoung; Mori Akio; Severson David W

Resumo: An endogenous meiotic driver in the dengue and yellow fever vector mosquito Aedes aegypti can cause highly male-biased sex ratio distortion in crosses from suitable genetic backgrounds. We previously selected a strain that carries a strong meiotic drive gene (D) linked with the male-determining allele (M) on chromosome 1 in A. aegypti. Here, we performed segregation analysis of the M(D) locus among backcross (BC(1)) progeny from a driver male and drive-sensitive females. Assessment of sex ratios among BC(2) progeny showed ?5.2% recombination between the M(D) locus and the sex determination locus. Multipoint linkage mapping across this region revealed consistent marker orders and recombination frequencies with the existing reference linkage map and placed the M(D) locus within a 6.5-cm interval defined by the LF159 locus and microsatellite marker 446GAA, which should facilitate future positional cloning efforts.

Imprenta: The Journal of Heredity, v. 103, n. 2, p. 303-307, 2012

Identificador do objeto digital: 10.1093/jhered/esr134

Descritores: Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2012