Fixation and preparation of developing tissues from Aedes aegypti.

Autor(es): Clemons Anthony; Haugen Morgan; Flannery Ellen; Kast Kristopher; Jacowski Caitlin; Severson David; Duman-Scheel Molly

Resumo: Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol describes a method for fixation and dissection of Ae. aegypti embryos, larvae, and pupae. Tissue processed in this manner can be used subsequently for in situ hybridization detection of mRNA or immunohistochemical analysis of protein expression.

Imprenta: Cold Spring Harbor Protocols, v. 10, 2010

Identificador do objeto digital: 10.1101/pdb.prot5508

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Molecular methods ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemic ; Aedes aegypti - Public health

Data de publicação: 2010