First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association.
Autor(es): Gusm?o Desiely Silva; Santos Ad?o Valmir; Marini Danyelle Cristine; Russo Erica de Souza; Peixoto Anelise Maria Dias; Bacci Júnior Maurício; Berbert-Molina Marília Amorim; Lemos Francisco José Alves
Resumo: We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
Palavras-Chave: Aedes aegypti; Gut diverticulum; Microbiota; Serratia sp.
Imprenta: Memo?rias do Instituto Oswaldo Cruz, v. 102, n. 8, p. 919-924, 2007
Identificador do objeto digital: http://dx.doi.org/10.1590/S0074-02762007000800005
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA
Data de publicação: 2007