Experimental inoculation of Artibeus jamaicensis bats with dengue virus serotypes 1 or 4 showed no evidence of sustained replication.

Autor(es): Cabrera-Romo Salomé; Recio-Tótoro Benito; Alcalá Ana C; Lanz Humberto; del Ángel Rosa María; Sánchez-Cordero Victor; Rodríguez-Moreno Ángel; Ludert Juan E

Resumo: Dengue is the most important mosquito-borne viral disease to humans. Bats are potential reservoirs for flaviviruses, including dengue virus (DENV). In this work, Artibeus jamaicensis bats were inoculated with two serotypes of DENV using different routes. For experimental inoculations (EI) 1 and 2, bats were inoculated subcutaneously or intraperitoneally with DENV-4; for EI-3 bats were inoculated intraperitoneally with DENV-1. Mock inoculated bats were kept as controls. In EI-4, bats were bitten by Aedes aegypti mosquitoes infected with DENV-1 or 4. Reverse transcription-polymerase chain reaction assays in plasma and spleen tissue collected from Day 1 to Days 9-17 after inoculation failed to reveal the presence of viral RNA in any of the samples. No evidence of circulating NS1 or specific anti-DENV IgG was detected in the plasma of the inoculated bats. These results indicate that A. jamaicensis bats are incapable of sustaining dengue virus replication and are unlikely to act as reservoirs for this virus.

Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 91, n. 6, p. 1227-1234, 2014

Identificador do objeto digital: 10.4269/ajtmh.14-0361

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Viral infections ; Aedes aegypti - virus ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2014