Development of an immunoassay for rapid detection of ganglioside GM(1) mimicry in Campylobacter jejuni strains
Autor(es): Prendergast M M,Kosunen T U,Moran A P
Resumo: Mimicry of peripheral nerve gangliosides by Campylobacter jejuni lipopolysaccharides (LPSs) has been proposed to induce cross-reacting antiganglioside antibodies in Guillain-Barré syndrome (GBS). Because current methods for LPS characterization are labor-intensive and inhibit the screening of large numbers of strains, a rapid GM(1) epitope screening assay was developed. Biomass from two agar plates of confluent growth yielded sufficient LPS using a novel phenol-water and ether extraction procedure. Extracts of LPS were reacted with cholera toxin (GM(1) ligand), peanut agglutinin (Gal beta1-->3GalNAc ligand), and anti-GM(1) antibodies. After the assay was validated, 12 of 59 (20%) C. jejuni serostrains, including four serotypes that have not previously been associated with GBS, reacted with two or more anti-GM(1) ganglioside reagents. Subsequently, LPS extracts from 5 of 7 (71%) C. jejuni isolates and 2 of 3 (67%) C. jejuni culture collection strains bore GM(1) structures. Overall, the assay system was reliable, efficient, and reproducible and may be adapted for large-scale epidemiological studies.
Imprenta: Journal of Clinical Microbiology, v. 39, n. 4, p. 1494-1500, 2001
Identificador do objeto digital: 10.1128/JCM.39.4.1494-1500.2001
Descritores: Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Molecular methods ; Guillain-Barre Syndrome - Molecular screening ; Guillain-Barre Syndrome - Immunology
Data de publicação: 2001