Detection of anti-ganglioside antibodies in Guillain-Barré syndrome and its variants by the agglutination assay
Autor(es): Alaedini Armin,Briani Chiara,Wirguin Itzhak,Siciliano Gabriele,D'Avino Carla,Latov Norman
Resumo: Sera from 40 patients with Guillain-Barré syndrome (GBS), including the subtypes acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), and Miller Fisher syndrome (MFS) were examined for the presence of anti-ganglioside antibodies using the ganglioside agglutination assay, and the enzyme-linked immunosorbent assay (ELISA). In the ELISA system, sera were tested for IgM and IgG antibodies to GM1, GM2, GD1a, GD1b, GT1b, and GQ1b gangliosides. Antibodies to gangliosides were detected in 21 (53%) of the GBS patients by agglutination assay and in 17 (43%) of the patients by ELISA. Some of the sera reacted with more than one ganglioside. Antibodies were not found in the control sera that were studied. The agglutination assay may be useful for rapid screening of GBS sera for antibodies to multiple gangliosides.
Palavras-Chave: Guillain-Barré syndrome; Acute inflammatory demyelinating polyneuropathy; Acute motor axonal neuropathy; Acute motor and sensory axonal neuropathy; Miller Fisher syndrome; Ganglioside antibodies
Imprenta: Journal of the Neurological Sciences, v. 196, n. 1-2, p. 41-44, 2002
Identificador do objeto digital: 10.1016/S0022-510X(02)00022-9
Descritores: Guillain-Barre Syndrome - Cell ; Guillain-Barre Syndrome - Cytopathology ; Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Serology ; Guillain-Barre Syndrome - Immunology ; Guillain-Barre Syndrome - Public health
Data de publicação: 2002
