Detection of antibodies in neuropathy patients by synthetic GM1 mimics

Autor(es): Pukin Aliaksei V,Jacobs Bart C,Tio-Gillen Anne P,Gilbert Michel,Endtz Hubert P,van Belkum Alex,Visser Gerben M,Zuilhof Han

Resumo: Antibodies to the ganglioside GM1 are associated with various forms of acute and chronic immune-mediated neuropathy, including Guillain-Barré syndrome (GBS) and multifocal motor neuropathy. In diagnostics and research, these antibodies are usually detected by GM1 preparations derived from bovine brain tissue, which are non-covalently attached to solid carriers such as enzyme-linked immunosorbent assay (ELISA) plates. Such brain-derived GM1 preparations are potentially contaminated with other glycolipids. In the current study, uncontaminated mono- and divalent synthetic analogs of the ganglioside GM1 were successfully attached via covalent bonds onto the surface of ELISA plates. The resulting modified diagnostic tool showed strong affinities and good specificities for binding of monoclonal mouse and human anti-GM1 antibodies and cholera toxin, as well as for the anti-GM1 antibodies in serum samples from neuropathy patients. While these proof-of-principle experiments reveal the potential of synthetic ganglioside mimics in diagnostics, they show the necessity of further studies to overcome certain limitations, specifically the non-specific interactions in the negative control assays with synthetic GM1.

Palavras-Chave: Antibody; Diagnostics; Ganglioside mimics; GM1 ganglioside; Neuropathy

Imprenta: Glycobiology, v. 21, n. 12, p. 1642-1650, 2011

Identificador do objeto digital: 10.1093/glycob/cwr093

Descritores: Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Immunology

Data de publicação: 2011