Autor(es): Godschalk Peggy C R,Heikema Astrid P,Gilbert Michel,Komagamine Tomoko,Ang C Wim,Glerum Jobine,Brochu Denis,Li Jianjun,Yuki Nobuhiro,Jacobs Bart C,van Belkum Alex,Endtz Hubert P

Resumo: Molecular mimicry of Campylobacter jejuni lipo-oligosaccharides (LOS) with gangliosides in nervous tissue is considered to induce cross-reactive antibodies that lead to Guillain-Barre syndrome (GBS), an acute polyneuropathy. To determine whether specific bacterial genes are crucial for the biosynthesis of ganglioside-like structures and the induction of anti-ganglioside antibodies, we characterized the C. jejuni LOS biosynthesis gene locus in GBS-associated and control strains. We demonstrated that specific types of the LOS biosynthesis gene locus are associated with GBS and with the expression of ganglioside-mimicking structures. Campylobacter knockout mutants of 2 potential GBS marker genes, both involved in LOS sialylation, expressed truncated LOS structures without sialic acid, showed reduced reactivity with GBS patient serum, and failed to induce an anti-ganglioside antibody response in mice. We demonstrate, for the first time, to our knowledge, that specific bacterial genes are crucial for the induction of anti-ganglioside antibodies.

Imprenta: The Journal of Clinical Investigation, v. 114, n. 11, p. 1659-1665, 2004

Identificador do objeto digital: 10.1172/JCI15707

Descritores: Guillain-Barre Syndrome - Biosynthesis ; Guillain-Barre Syndrome - Genome ; Guillain-Barre Syndrome - Molecular Structure ; Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Infectious diseases ; Guillain-Barre Syndrome - Immunology

Data de publicação: 2004

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